这段英文不会翻译了 生物化学类的

The development of protein purification techniques has
always been an important issue over the decades. Affinity
adsorption, one of the protein purification methods, is frequently
used because of its binding specificity to the target
compound. It could be applied to three different kinds of
separations such as affinity adsorption, affinity precipitation,
and affinity partition according to different requirements [1].
Affinity adsorption is a novel separation method based on the
specific binding of protein to the adsorbent. Three applications
from the approach of affinity adsorption, classified by
their bioselectivities, can be developed such as affinity ligands,
immuno-affinity, and receptors. Affinity ligands may
include enzyme substrates, enzyme inhibitors, dyes, heavy
metal ions, and so forth. Starch, the substrate of α-amylase,
either cross-linked or not, was used as one of the adsorbents
for the isolation of amylases from wild strains or recombinant strains [2–4]. Various starch treatments can be applied
such as adding ethanol or salt into amylase solution,
and heat–moisture treatment, to enhance the adsorption of
amylase [5]. However, one major defect of applying starch
material to the affinity adsorption is the easy hydrolysis of
starch by the amylolytic enzyme mixture. Besides, it takes a
longer time to reach adsorption equilibrium when compared
with other polysaccharide materials. Alginate, a non-toxic
and cheap polysaccharide of marine origin, was used to precipitate
α-amylase from sweet potato with 80% activity yield
and 44-fold purification [6]. Agarose gel, a material often
used in affinity chromatography, was activated by epichlorohydrin
or bifunctional epoxides and was thus capable of
binding with substances containing amino groups. Cyclohexaamylose
(CHA) was used as the ligand.
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榛子 5级
2009-02-18 回答
The development of protein purification techniques has
always been an important issue over the decades
几十年来,蛋白纯化技术的发展一直倍受关注。
Affinity adsorption, one of the protein purification methods, is frequently
used because of its binding specificity to the target
compound.
亲和吸附,蛋白纯化的一种方法,由于其与目标化合物的结合特异性而经常被使用。